Vol. XXX Issue 2


December 2019

ISSN online version: 1852-6233
DOI: 10.35407/bag.2019.30.02

ARTICLE 1 - research

MOLECULAR MARKER ANALYSIS OF SPIKE FERTILITY INDEX AND RELATED TRAITS IN A BREAD WHEAT RECOMBINANT INBRED LINE POPULATION

ANÁLISIS DE MARCADORES MOLECULARES PARA EL ÍNDICE DE FERTILIDAD DE ESPIGA Y CARACTERES ASOCIADOS EN UNA POBLACIÓN DE LÍNEAS ENDOCRIADAS RECOMBINANTES DE TRIGO PAN.

Panelo J.S. , Alonso M.P., Mirabella N.E. , Pontaroli A.C.

Spike fertility index (SF) has been well established as an ecophysiological trait related to grain number per unit area and a promising selection target in wheat breeding programs. Scarce information on the molecular basis of SF is available thus far. In this study, a preliminary molecular marker analysis was carried out in a RIL population derived from the cross between two Argentinean cultivars with contrasting SF to identify candidate genomic regions associated with SF. Twenty-four microsatellites and two functional markers that had been found to co-segregate with SF in a bulked-segregant analysis of the F3 generation of the population were analyzed. Phenotypic data were collected from three field experiments carried out during 2013, 2014 and 2015 growing seasons at Balcarce, Argentina. Two genomic regions associated with SF in chromosomes 5BS and 7AS were detected, which merit further investigation.

Key words: selection, genomic regions, grain number, yield, QTL, spike fertility index, fruiting efficiency
Doi: 10.35407/bag.2019.xxx.02.01
Language:
English

ARTÍCLE 2 - research

KARYOLOGICAL STUDY IN THE CHILEAN RHATANY Krameria cistoidea HOOK. & ARN. (KRAMERIACEAE)

ESTUDIO CARIOLÓGICO EN EL PACUL CHILENO Krameria cistoidea HOOK. & ARN. (KRAMERIACEAE)

Palma Rojas C., Jara Seguel P., García M., von Brand E., Araya Jaime C.

The karyotype of the plant species Krameria cistoidea Hook. & Arn. was studied by assessing chromosome characters such as morphology, size, and C-banding pattern. The karyotype of K. cistoidea was composed only by metacentric chromosomes in the two populations studied. The haploid set length was 51.2.3 µm and the mean chromosome size was 8.60.78 µm. Some similarities in chromosome morphology and size can be observed among K. cistoidea and K. triandra, in addition to the chromosome number 2n=12 which is conserved within the genus. K. cistoidea exhibited a symmetric banding pattern with large C-bands in the telomeres of the short and long arms of all chromosomes, except the short arm of pair 1. The relative length of the C-bands was 23.5% of the total haploid set length. These cytological results on K. cistoidea are the first data on quantitative karyotype morphology and C-banding patterns in the genus Krameria.

Key words: Krameria, karyotype, C-banding
Doi: 10.35407/bag.2019.xxx.02.02
Language: English

ARTÍCLE 3 - review

TP53 PATHOGENIC VARIANTS RELATED TO CANCER

VARIANTES PATOGÉNICAS DE TP53 RELACIONADAS CON CÁNCER

Rosero C.Y., Mejia L.G., Corredor M.

TP53 or P53 is a tumor suppressor gene known as the “genome guardian”, responsible for inducing cell response to DNA damage, by stopping the cell cycle in case of mutation, activating DNA repair enzymes, initiating senescence and activation of apoptosis. Mutations in the gene sequence can cause non-synonymous mutations or errors in the reading frame by insertion, deletion or displacement of nucleotides: e.g., c.358A>G mutation in exon 4 and variants located in exons 9 and 10 of the TD domain. Therefore, in this review, we will see that changes in the reading frame, including the loss of one or two base pairs could prevent accurate transcription or changes in the structure and function of the protein, and could completely impair reparation function. These changes promote self-sufficiency in growth signaling, insensitivity to anti-growth signals, and evasion of apoptosis, resulting in limitless replication and induction of metastatic angiogenesis, generating as a consequence the proliferation of tumor, neoplastic, and lymphoid cells. Taking into account the importance of TP53 in the regulation of the cell cycle, the objective of this review is to update information related to the role of this gene in the development of cancer and the description of genetic variations.

Key words: Neoplasms, nuclear phosphoprotein p53, Tumor Suppressor, mutation, Clinvar, Uniprot
Doi: 10.35407/bag.2019.xxx.02.03
Language: English

ARTÍCLE 4 - review

THE HUMAN VARIOME PROJECT COUNTRY NODE OF ARGENTINA IN THE FIRST TWO YEARS OF ACTIVITY: PAST, PRESENT AND FUTURE

EL NODO ARGENTINO DEL PROYECTO VARIOMA HUMANO EN LOS PRIMEROS DOS AÑOS DE ACTIVIDAD: PASADO, PRESENTE Y FUTURO

Solano A.R., Garrido M., Mele P.G., Podestá E.J., Reichardt J.K.V.

The Human Variome Project (HVP) is an international effort aiming systematically to collect and share information on all human genetic variants. It has been working for years in collaboration with local scientific societies by establishing systems to collect every genetic variant reported in a country and to store these variants within a database repository: LOVD (Argentinian chapter: ar.lovd.org). Formally established in 2017 in the Argentinian Node, up to June 2019 we collected more than 25,000 genetic variants deposited by 17 different laboratories. Nowadays the HVP country nodes represent more than 30 countries. In Latin America there are four country nodes: Argentina, Brazil, Mexico and Venezuela; the first two interacted recently launching the LatinGen database. In the present work we want to share our experience in applying the HVP project focusing on its organization, rules and nomenclature to reach the goal of sharing genetic variants and depositing them in the Leiden Open Variation Database. Contributing laboratories are seeking to share variant data to gain access all over the country. It is one of our goals to stimulate the highest quality by organizing courses, applying current nomenclature rules, sponsoring lectures in national congresses, distributing newsletter to serve the Argentinian genomics community and to stimulate the interaction among Latin America countries.

Key words: Data sharing, Argentinian chapter of LOVD, Human Variome Project (HVP)-Argentina
Doi: 10.35407/bag.2019.xxx.02.04
Language: English

ARTÍCLE 5 - research

GENOME SIZE IN THREE SPECIES OF Glandularia AND THEIR HYBRIDS

TAMAÑO DEL GENOMA EN TRES ESPECIES DE Glandularia Y SUS HÍBRIDOS

Ferrari M.R., Greizerstein E.J., Poggio L.

In this work the relationship between genome size of Glandularia species and the meiotic configurations found in their hybrids are discussed. Glandularia incisa (Hook.) Tronc., growing in two localities of Corrientes and Córdoba provinces, Argentina, with different ecological conditions, showed inter-population variability of the 2C-value. The DNA content found in the Corrientes locality (2.41 pg) was higher than that obtained in the Córdoba locality (2.09 pg) which has more stressful environmental conditions than the former. These values are statistically different from those that were found in Glandularia pulchella (Sweet) Tronc. from Corrientes (1.43 pg) and in Glandularia perakii Cov. et Schn from Córdoba (1.47 pg).
The DNA content of the diploid F1 hybrids, G. pulchella × G. incisa and G. perakii × G. incisa, differed statistically from the DNA content of the parental species, being intermediate between them. Differences in the frequency of pairing of homoeologous chromosomes were observed in the hybrids; these differences cannot be explained by differences in genome size since hybrids with similar DNA content differ significantly in their meiotic behavior. On the other hand, the differences in the DNA content between the parental species justify the presence of a high frequency of heteromorphic open and closed bivalents and univalents with different size in the hybrids.

Key words: Intra-specific DNA content variability, homoeologous pairing, heteromorphic bivalents
Doi: 10.35407/bag.2019.xxx.02.05
Language: English

OBITUARY

DR. ROBERTO COCO

CONGRESO ALAG 2019.
6 AL 9 DE OCTUBRE. MENDOZA, ARGENTINA.

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